Plasmid_Backbone

Part:BBa_J176122:Design

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-10-21)

pcDNA3.1+ puro


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal BglII site found at 12
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 2148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 3673
    Illegal SapI site found at 2593


Design Notes

1. Deleting the CMV promoter via SpeI 2. Replcacing the neomycin resistance gene with a puromycin resistance gene

Source

TBA

References